Molecular Diagnostics

Supporting agriculture and horticulture in a number of ways through diagnostic services based on polymerase chain reaction (PCR) analyses.

Sector: Crop Science

James Hutton Limited can support agriculture and horticulture in a number of ways through diagnostic services based on polymerase chain reaction (PCR) analyses.  Molecular Diagnostic tests can detect specific sequences in plant DNA or RNA that may be associated with disease resistance, quality/agronomic traits and varietal issues.  These diagnostic tests can be applied to early generation breeding material or developed varieties using a range of tissue types.  James Hutton Limited can offer a range of diagnostics for soft fruit, potato, barley and soil.

Molecular Diagnostic can be used for:

  • Screening germplasm for molecular markers that have been identified at James Hutton Institute and can be used to suggest possible traits in plant material
  • Validating new molecular markers
  • Test high health plant material for viruses and other diseases 
  • Authenticity testing for both propagators of plant material and nurseries buying in plant material
  • Analysis of soils for the presence of free living nematodes

Molecular Diagnostics for Soft Fruit

Screening of germplasm covering the Rubus genus (particularly raspberry and blackberry) using molecular markers associated with key traits for pest and disease resistance and fruit quality:

  • Raspberry root rot resistance 
  • Fruit size 
  • Validation phase of fruit softening
  • Validation phase of fruit yield

We also have the capability to screen a range of other Rubus traits, including architectural.

Screening of germplasm covering the Ribes genus (currants):
Gall mite resistance

DNA Fingerprinting
This provides a DNA profile of the variety.  Genotyping services are available for both soft fruit varieties and potato.  The generation of DNA fingerprints can be used for a variety of purposes.

  • Trueness-to-type
  • Varietal issues/mix-ups
  • Relatedness of breeding material/phylogenetic analysis (primarily for soft fruit)

James Hutton Limited also has an extensive database of DNA profiles of soft fruit varieties for comparative purposes.

Molecular Diagnostics for Barley

Molecular Markers
Resistance to Barley Yellow Mosaic Virus
Test for presence/absence of cyanogenic compound epiheterodendrin (EPH) in malting barley

Molecular Diagnostics for Potatoes

Molecular Markers
H1 Gene
Validation phase of marker GPA5, gene resistance to white PCN of S.Vernei origin
PMTV

Conventional Testing
Canister testing for White and Golden PCN

DNA Fingerprinting
This provides a DNA profile of the variety.  Genotyping services are available for both soft fruit varieties and potato.
The generation of DNA fingerprints can be used for a variety of purposes.

Molecular Diagnostics for Soil

James Hutton Limited can offer both conventional, microscopic testing and a recently developed diagnostic test to identify the presence of Free Living Nematodes in soil.
This diagnostic provides a rapid and cost effective identification of the four most common Paratrichodorus and Trichodorus species in the UK, known to vector TRV. Combined with an appropriate sampling strategy, the diagnostic offers growers an opportunity to generate a robust field map of the distribution of FLN species capable of transmitting TRV.

Other Services