Special Offer for June/July 2018
Molecular diagnostic tests detect specific sequences in plant DNA that may be associated with disease resistance, quality/agronomic traits and varietal issues. They can be applied to early generation breeding material or developed varieties.
James Hutton Limited can offer two molecular diagnostic tests for barley:
Resistance to Barley Yellow Mosaic Virus (BaYMV) and Barley Mild Mosaic Virus (BaMMV)
James Hutton Limited screens barley lines/varieties for resistance to BaMMV and two strains of BaYMV using a fluorescently labelled molecular marker, Bmac29. The test is carried out alongside reference DNA samples from cultivars known to be susceptible, rym4 resistant (BaMMV and BaYMV-1) or rym 5 resistant (BaMMV, BaYMV-1 and BaYMV-2). Resulting DNA profiles are analysed to determine their resistance status.
Presence or Absence of EPH in Malting Barley
An EPH genotyping test is used to identify whether a barley line/variety is a producer or non-producer of the cyanogenic compound epiheterodendrin (EPH), an undesirable cyanogenic compound found in malting barley.
To ensure accuracy of results, a duplicate test is carried out. DNA is screened using a multiplex marker along with reference samples from known EPH producers/non-producers using polymerase chain reaction technology and results analysed using gel electrophoresis.
Costs per sample for testing from barley seed, based on testing between 1 and 50 samples. Please enquire for >50 samples. Minimum service charge of £300.
BaYMV/BaMMV resistance test |
Price (exc. VAT) |
Unreplicated test |
£60 |
Replicated test |
£83 |
EPH test |
Price (exc. VAT) |
Replicated test |
£53.50 |
Additional Services |
Price (exc. VAT) |
DNA extraction from seed |
£19 |
DNA extraction from leaf material |
£11 |
Genotyping/PCR only services are also available, price is dependent on the number of samples to be processed. |
A comprehensive report will be provided on completion of analysis and results presented in an easy to read table. Should any follow up testing be required, James Hutton Limited will retain DNA samples in cold storage for two years.
As well as barley, James Hutton Limited offers a range of diagnostics for soft fruit, potatoes and soil.
For more information, contact Vanessa Young (below).